Development of Aptamer Based Biosensors

Aptamers are DNA or RNA molecules, which are capable of binding different classes of targets with high affinity and specifity. They bind to the target with high selectivity and specifity because of their specific three-dimensional shape. Binding capacity is the result of oligonucleotides’ three-dimensional conformation not nucleotide base-base complementarity.


Confocal flourescence and light image of trypsine coated magnetic beads treated with round 7 of SELEX library (a, b) and treated with non-selected begining library(c,d).


Aptamers are produced and selected with the use of combinatorial chemistry, technique named in vitro selection, in vitro evolution or SELEX (Systematic Evolution of Ligands by EXponential enrichment).


Carbon Nanotube Based Biosensors

Confocal flourescence and light image of  target cells that is labelled with selected aptamer libraries.

Carbon nanotubes are rolled-up sheets of carbon atoms that can be narrower than 1 nanometer in diameter. A nanometer is one millionth of a millimeter, or the span of 10 hydrogen atoms.

The sensors could eventually be used to monitor biochemical changes in biological fluids and tissue in real time, according to the researchers.



Nanoparticle Based Biosensors


QCM Based Detection Systems