Middle East Technical University, Department of Biological Sciences
 

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BTECH704
BIO417

NEW GENERATION DIAGNOSIS SYSTEMS FOR GMO DETECTION

 

Nanoparticle Based Biosensors

QCM Based Detection Systems

PLANT GENOMICS VIA MICROARRAY ANALYSIS

 

Transcriptome Analysis of Crop Plants Under Abiotic Stress Conditions

Supplementary Material for the manuscript entitled Microarray
Analysis of Late Response to Boron Toxicity in Barley (Hordeum vulgare L.) Leaves

GENE EXPRESSION LEVEL ANALYSIS UNDER ABIOTIC STRESS CONDITIONS

 

Stress Types: Salinity, Drought, Cold

Target Plants: Wheat, Barley

Target Genes: Superoxide dismutase gene, Trehalose-6-phosphate synthase gene, Trehalase gene

Techniques: Northern Blot, Quantitative Real Time PCR (Q-PCR), Reverse Transcriptase PCR (RT-PCR)

 

GENETIC MANIPULATION OF CROP PLANTS

Target Plant Species

Tobacco (model)

Potato

Wheat

Lentil

Chickpea

Sugarbeet

Target Explants

Mature-immature embryo & infloresences

Cotyledonary nodes

Leaf, cotyledon, stem

Transformation Techniques

Agrobacterium mediated

Microprojectile bombardment (Wheat, Lentil)

Interested Agronomic Characters

Abiotic stress resistance (salt & drought - P5CS, proline, aldose reductase, STO, CBF1, transcription factors)

Microbial resistance (pflp)

Delayed ripening (ACC oxidase, antisense)

 

STRESS PHYSIOLOGY & BIOCHEMISTRY

 

Physiological and biochemical aspects of abiotic stress resistance in crop plants

Stress Types: boron toxicity, heat, cold, salt, drought, cadmium toxicity

Target Plant: wheat, lentil, barley, sunflower, chickpea

Techniques: 1D-2D Electrophoresis, Photosystem-II Activity Measurements, Membrane Leakage Tests (CMS), TTC Viability Tests, Kinetic Analysis of Antioxidant Defense System Enzymes (SOD, Ascorbate peroxidase, Catalase, Glutathion reductase), H2O2, MDA, Proline measurements.

 

MICROTUBER PRODUCTION IN POTATO

Major aim is to produce microtubers tubers from cultured potato shoots using bioreactors and conventional tissue culture methods.


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2002 METU Plant Biotech Group. All rights reserved.

Middle East Technical University, Department of Biological Sciences Lab10 Ankara/TURKEY

Tel. (312) 210 51 71 Fax. (312) 210 12 89

Designed By

.A. "Changes in Total Protein