OF MICROTUBERS IN POTATO
Department of Biology
Assoc. Prof. Dr. Hüseyin Avni Öktem
1999, 70 pages
(Solanum tuberosum L.) is one of the major food sources and
is being cultivated in all parts of Turkey. Potato is produced
vegetatively by using seed tubers, which should be true to type and
free of diseases.
studies demonstrate the potential use of microtubers as seed tubers.
Microtuber is an alternative end product of micropropagation,
produced by allowing in vitro plantlets to grow under tuber
the present study microtuberization potential of three potato
cultivars (Ausonia, Granola, Marfona) that are commonly cultivated
in Turkey were investigated. Among the studied microtuberization
systems, two-phase solid propagation-liquid tuber induction system
was found to be the best yielding system. This system yielded
microtubers with average weights of 192, 92 and 82 mg and average
number of microtubers per tube were 2, 3 and 1 for cvs Ausonia,
Granola and Marfona, respectively. Addition of charcoal
significantly (p<0.05) increased the weight of microtubers (310,
132 and 111 mg for cvs Ausonia, Granola and Marfona, respectively)
where number of microtubers per tube was not affected. Except cv
Marfona, most of the microtubers retained 80% of their fresh weight
after eight weeks of storage at 4°C
Storage temperature and time were found to influence the sprouting
ability of the microtubers. At 4°C,
after 16 weeks of storage, 48, 44 and 0% of microtubers of cvs
Ausonia, Granola and Marfona were sprouted, respectively, whereas at
after 12 weeks, for the same cultivars the sprouting percentages
were 100, 29 and 19%. Sprouted microtubers were found to be suitable
for both in vitro culturing and direct cultivation in soil.
conclusion, with respect to weight and number of produced
microtubers, the described two phase microtuber induction method
seems to be one of the best yielding microtuberization systems
described in literature.
words: Potato, in vitro tuberization, microtuber, two-phase
induction system, charcoal.
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